Anti-Alpha-tubulin (Phospho-Y272) Antibody

SKU: orb665690

Description

Rabbit polyclonal antibody to Alpha-tubulin (Phospho-Y272)

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IH, WB
• Dilution Range: WB: 1-500-1000
• Reactivity: Human, Mouse, Primate, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y272 of human Alpha-tubulin protein. The exact sequence is proprietary.
• Target: TUBA1A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-TUBA1A antibody, anti-TUBA3 antibody, anti-Tubulin alpha-1A chain antibody, anti-Alpha-tubulin 3 antibody, anti-Tubulin B-alpha-1 antibody, anti-Tubulin alpha-3 chain antibody, anti-TUBA1B antibody, anti-Tubulin alpha-1B chain antibody, anti-Alpha-tubulin ubiquitous antibody, anti-Tubulin K-alpha-1 antibody, anti-Tubulin alpha-ubiquitous chain antibody, anti-TUBA1C antibody, anti-TUBA6 antibody, anti-Tubulin alpha-1C chain antibody, anti-Alpha-tubulin 6 antibody, anti-Tubulin alpha-6 chain antibody, anti-TUBA3C antibody, anti-TUBA2 antibody, anti-TUBA3D antibody, anti-Tubulin alpha-3C/D chain antibody, anti-Alpha-tubulin 2 antibody, anti-Alpha-tubulin 3C/D antibody, anti-Tubulin alpha-2 chain antibody, anti-TUBA3E antibody, anti-Tubulin alpha-3E chain antibody, anti-Alpha-tubulin 3E antibody, anti-TUBA4A antibody, anti-TUBA1 antibody, anti-Tubulin alpha-4A chain antibody, anti-Alpha-tubulin 1 antibody, anti-Testis-specific alpha-tubulin antibody, anti-Tubulin H2-alpha antibody, anti-Tubulin alpha-1 chain antibody

Western blot analysis of Alpha-tubulin (Phospho-Y272) expression in U87MG (A), A375 (B), Hela (C), A2780 (D), EC9706 (E), C6 (F), CT26 (G) whole cell lysates. (Predicted band size: 50; 49 kD; Observed band size: 52 kD)

Immunohistochemical analysis of Alpha-tubulin (Phospho-Y272) staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

TUBA1A

UniProt

P68373, P68369, Q6AYZ1, P68363, P05213, Q71U36, Q13748, P68370, Q9BQE3, P68366, Q6PEY2, Q6P9V9

NCBI Gene ID

7277, 112714, 100909441, 7278, 22142, 64158, 113457, 10376, 500929, 84790, 22143, 22146, 7846