Anti-alpha-Tubulin Purified

SKU: orb738510

Description

Mouse monoclonal antibody to TUBA1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution range: ICC: 4-8 μg/ml, WB: 1 μg/ml
• Reactivity: Aves, Human, Mammal, Mouse, Rat, Yeast
• Application Notes:
  Immunocytochemistry: Recommended dilution 4-8 μg/ml.Western blotting: Recommended dilution 1-2 μg/ml; reducing conditions.Flow cytometry: Recommended dilution: 8-12 μg/ml; intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone No.: YOL1/34
• Immunogen: Yeast tubulin
• Target: alpha-Tubulin
• Purity: Purified by protein-G affinity chromatography.
• Purification: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-TUBA antibody

Immunocytochemistry staining of alpha-tubulin in K562 cells using purified rat monoclonal antibody YOL1/34 (concentration in sample 6 μg/ml, DAR FITC, left picture) vs. Hoechst 34580 nuclear staining (right picture).

Immunocytochemistry staining of alpha-tubulin in K562 cells using purified rat monoclonal antibody YOL1/34 (concentration in sample 6 μg/ml, DAR FITC, left picture) vs. Hoechst 34580 nuclear staining (right picture).

Quick Database Links

Gene Symbol

alpha-Tubulin

UniProt

Q71U36

NCBI Gene ID

7277