Atg4A Monoclonal Antibody

SKU: orb548419

Description

Anti-Atg4A Monoclonal Antibody. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Images & Validation

• Tested Applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB 1:500-1:2000IHC 1:50-1:200IP 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: ACHB-1
• Immunogen: A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.
• Molecular Weight: 45 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Death domain-containing protein CRADD; Caspase and RIP adapter with death domain; RIP-associated protein with a death domain; CRADD; RAIDD

Quick Database Links

UniProt

Q8WYN0