Anti-BCL3 Antibody

SKU: orb666129

Description

Rabbit polyclonal antibody to BCL3

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide of human BCL3. The exact sequence is proprietary.
• Target: BCL3
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-BCL4 antibody, anti-D19S37 antibody, anti-B-cell lymphoma 3 protein antibody, anti-BCL-3 antibody, anti-Proto-oncogene BCL3 antibody

Western blot analysis of BCL3 expression in A549 (A), mouse liver (B) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 60 kD)

Immunofluorescent analysis of BCL3 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

BCL3

UniProt

P20749, Q9Z2F6

NCBI Gene ID

12051, 602