Anti-Beta-actin Antibody

SKU: orb393347

Description

Rabbit polyclonal antibody to ACTB.

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1:1000:3000, IHC-P: 1:200:500, IF/ICC: 1:100:200
• Reactivity: Human, Mouse, Other, Primate, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein corresponding to human Beta-actin.
• Target: ACTB
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Actin cytoplasmic 1; Beta-actin

Western blot analysis of Beta-actin expression in HepG2 (A), 293T (B), mouse kidney (C), rat liver (D), rabbit testis (E), sheep lung (F) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 42 kD)

Immunohistochemical analysis of Beta-actin staining in human ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Beta-actin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

ACTB

UniProt

P60709, P60710, P60711

NCBI Gene ID

60, 81822, 11461