Anti-Caspase 1 p10 Antibody

SKU: orb213638

Description

Rabbit polyclonal antibody to Caspase 1

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1-500-1-1000, IF/ICC: 1-100-1-500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Caspase 1. The exact sequence is proprietary.
• Target: CASP1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-CASP-1 antibody, anti-CASP1 antibody, anti-CASP1_HUMAN antibody, anti-Caspase 1 antibody, anti-Caspase-1 subunit p10 antibody, anti-p45 antibody

Western blot analysis of Caspase 1 p10 expression in human spleen (A) whole cell lysates. (Predicted band size: 45 kD; Observed band size: 45 kD)

Western blot analysis of Caspase 1 p10 expression in H1792 (A), MCF7 (B), Hela (C), mouse spleen (D), mouse lung (E), rat spleen (F) whole cell lysates.

Immunofluorescent analysis of Caspase 1 p10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

CASP1

UniProt

P29452, P43527, P29466

NCBI Gene ID

12362, 834