Anti-CD107b Antibody

SKU: orb1474002

Description

Mouse monoclonal antibody to CD107b

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/1000 - 1/2000)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Recombinant fusion protein of human CD107b. The exact sequence is proprietary.
• Target: LAMP2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lysosome-associated membrane glycoprotein 2; LAMP-2; Lysosome-associated membrane protein 2; CD107 antigen-like family member B; CD107b

Western blot analysis of CD107b expression in HeLa (A), T47D (B), Jurkat (C) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 96 kD)

Immunofluorescent analysis of CD107b staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

LAMP2

UniProt

P13473

NCBI Gene ID

3920