Anti-CD11c Antibody

SKU: orb665908

Description

Rabbit polyclonal antibody to CD11c

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1-500-2000, IHC-P: 1-50-200
• Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein of human CD11c. The exact sequence is proprietary.
• Target: ITGAX
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-CD11C antibody, anti-Integrin alpha-X antibody, anti-CD11 antigen-like family member C antibody, anti-Leu M5 antibody, anti-Leukocyte adhesion glycoprotein p15095 alpha chain antibody, anti-Leukocyte adhesion receptor p15095 antibody, anti-CD11c antibody

Western blot analysis of CD11c expression in THP1 (A), mouse spleen (B) whole cell lysates. (Predicted band size: 127 kD; Observed band size: 150 kD)

Immunohistochemical analysis of CD11c staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CD11c staining in THP1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

ITGAX

UniProt

P20702, Q9QXH4

NCBI Gene ID

3687, 16411