Anti-CD147 [OX-114]

SKU: orb2645685

Description

Anti-CD147 [OX-114]

Research Area

,immunology; adaptive immunity; CD markers; cancer; ECM; neurogenesis; carbohydrate metabolism.

Images & Validation

• Tested Applications: FC, IHC, IP, WB
• Reactivity: Mouse

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: OX-114
• Immunogen: OX-114 was generated by immunizing rats with recombinant mCD147d0-2rCD4d3+4.
• Target: CD147
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Basigin; basic immunoglobulin superfamily; HT7 antigen; membrane glycoprotein gp42; gp42; gp-42; OX114; MRC OX-114; MRC OX114

Flow-cytometry using the anti-CD147 antibody OX-114 NIH3T3 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of OX-114 (orb2645685, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed NIH3T3 cells and mouse splenocytes with anti-CD147 antibody OX-114. Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells (A) and mouse splenocytes (B) permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of OX-114 (orb2645685) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels in A and B show from left-right, top-bottom orb2645685, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CD147 antibody OX-114 Mouse liver lysate (A), mouse brain lysate (B) and mouse heart lysate (C) samples (35 µg protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of OX-114 (orb2645685) at 2, 2 and 1 µg/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CD147 is 42.4kDa.

Quick Database Links

Gene Symbol

CD147

UniProt

P18572