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Description
Research Area
Images & Validation
−| Tested Applications | IF, IP, WB |
|---|---|
| Dilution Range | WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IP (1/10 - 1/100) |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Clone No. | SIRPA |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Anti-CD172a Antibody [orb215378]
IH, WB
Human, Mouse, Primate, Rat
Rabbit
Polyclonal
Unconjugated
30 μl, 100 μl, 200 μlAnti-CD172a/b Antibody [orb515259]
IF, IH, WB
Human
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 30 μl

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Immunofluorescent analysis of CD172a staining in THP1 Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of CD172a expression in Hela (A), A549 (B), C6 (C), CT26 (D) whole cell lysates.
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UniProt Details
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Protocol Information
Anti-CD172a Antibody (orb1423193)
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