Anti-CD178 Antibody

SKU: orb248894

Description

Mouse monoclonal antibody to CD178

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: FC, IF
• Reactivity: Human
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: NOK-1
• Immunogen: Native purified human CD178.
• Target: FASLG
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FASLG antibody, anti APT1LG1 antibody, anti CD95L antibody, anti FASL antibody, anti TNFSF6 antibody, anti Tumor necrosis factor ligand superfamily member 6 antibody, anti Apoptosis antigen ligand antibody, anti APTL antibody, anti CD95 ligand antibody, anti CD95-L antibody, anti Fas antigen ligand antibody, anti Fas ligand antibody, anti FasL antibody, anti CD antigen CD178) antibody

Quick Database Links

Gene Symbol

FASLG

UniProt

P48023

NCBI Gene ID

356