Anti-CD185 Antibody-APC labled

SKU: orb1294110

Description

Mouse monoclonal antibody against CD185 conjugated to APC

Research Area

Product Categories/Research Area,Signaling Pathways/GPCR,Product Categories/Antibodies,Neuroscience,Signaling Pathways,Epigenetics,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: FC, IF
• Dilution Range: 10 μl / assay
• Reactivity: Human
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: MU5UBEE
• Immunogen: Human CXCR5-transfected cells
• Target: CXCR5
• Conjugation: APC

Storage & Handling

• Storage: Shipped and store at 4°C for one year. Do not freeze.
• Buffer/Preservatives: Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BLR1; MDR15; C-X-C chemokine receptor type 5; CXC-R5; CXCR-5; Burkitt lymphoma receptor 1; Monocyte-derived receptor 15; MDR-15; CD185

Quick Database Links

Gene Symbol

CXCR5

UniProt

P32302

NCBI Gene ID

643