Anti-CD20 Reference Antibody (rituximab)

SKU: orb1818449

Description

Recombinant Antibody

Images & Validation

• Tested Applications: ELISA, FC
• Reactivity: Human, Monkey

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG1
• Source/Expression System: CHO Cell
• Protein Sequence: CD20
• Molecular Weight: 145.2
• Endotoxin: < 0.001EU/μg,determined by LAL method.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20

The purity of Anti-CD20 Reference Antibody (rituximab) is more than 98.97%, determined by SEC-HPLC.

Immobilized human EGFR His at 2 µg/mL can bind Anti-CD20 Reference Antibody (rituximab), EC50=0.01573/0.01258 µg/mL

Anti-CD20 Reference Antibody (rituximab) -induced CDC activity was evaluated using Raji Cell. The max Lysis rate was approximately 74%.

Anti-CD20 Reference Antibody (rituximab) -induced ADCC activity was evaluated using Raji Cell. The max Lysis rate was approximately 35%.

Anti-CD20 Reference Antibody (rituximab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

Raji cells were stained with Anti-CD20 Reference Antibody (rituximab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC87=1.334 µg/mL

Quick Database Links

UniProt

P11836