Anti-CD23 Antibody

SKU: orb248902

Description

Mouse monoclonal antibody to CD23

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: FC, IF
• Reactivity: Human
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: EBVCS-5
• Immunogen: Native purified human CD23.
• Target: FCER2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FCER2 antibody, anti CD23A antibody, anti CLEC4J antibody, anti FCE2 antibody, anti IGEBF antibody, anti Low affinity immunoglobulin epsilon Fc receptor antibody, anti BLAST-2 antibody, anti C-type lectin domain family 4 member J antibody, anti Fc-epsilon-RII antibody, anti Immunoglobulin E-binding factor antibody, anti Lymphocyte IgE receptor antibody, anti CD antigen CD23) antibody

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD23 Antibody, followed by anti-mouse IgG PE.

Quick Database Links

Gene Symbol

FCER2

UniProt

P06734

NCBI Gene ID

2208