Anti-CD247 Antibody

SKU: orb1474746

Description

Rabbit polyclonal antibody to CD247

Images & Validation

• Tested Applications: IH, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/200)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD247. The exact sequence is proprietary.
• Target: CD247
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD247

Western blot analysis of CD247 expression in HuT78 (A), mouse spleen (B), mouse lung (C), rat spleen (D), rat lung (E) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 16; 32 kD)

Immunohistochemical analysis of CD247 staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

CD247

UniProt

P24161, P20963

NCBI Gene ID

919, 12503