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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, FC |
|---|---|
| Dilution Range | ELISA 1:5000-10000|Flow Cyt 1:100 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Biosimilar Antibody |
|---|---|
| Host | Human |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target | CD27 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Powder |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−PE-conjugated Anti-CD27 (varlilumab biosimilar) mAb [orb2670868]
FC
Human
Human
Monoclonal
Unconjugated
100 TBiotinylated Anti-CD27 (varlilumab biosimilar) mAb [orb2321928]
ELISA, FC
Human
Human
Monoclonal
Unconjugated
50 μg, 100 μg

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ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, hFc tagged protein (orb689459) can bind Anti-CD27 Neutralizing antibody in a linear range of 3.2-80.0 ng/ml. In order to specifically detect orb689323, mouse anti-human Fab-specific antibody was used as detection antibody.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD27, mFc-His tagged protein orb689374 can bind Anti-CD27 Neutralizing antibody in a linear range of 0.64-16.0 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human CD27 protein (A) were surface stained with anti-CD27 neutralizing antibody 1 μg/ml (varlilumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.
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Protocol Information
Anti-CD27 (varlilumab biosimilar) mAb (orb689323)
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