Anti-CD273 Antibody

SKU: orb1473692

Description

Mouse monoclonal antibody to CD273

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/1000 - 1/4000), IH (1/50 - 1/200)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD273. The exact sequence is proprietary.
• Target: PDCD1LG2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B7DC; CD273; PDCD1L2; PDL2; Programmed cell death 1 ligand 2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; CD273

Western blot analysis of CD273 expression in Nontransfected 293T (A), Transfected PDL2transfected 293T (B) whole cell lysates. (Predicted band size: 30 kD; Observed band size: 40 kD)

Immunocytochemistry analysis of CD273 staining in PD-L2-transfected 293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a HRP-conjugated secondary antibody in PBS at room temperature. DAB was used as the chromogen.

Quick Database Links

Gene Symbol

PDCD1LG2

UniProt

Q9BQ51

NCBI Gene ID

80380