Anti-CD279 Antibody

SKU: orb705621

Description

Rabbit polyclonal antibody to PD1

Research Area

Immunology,Product Categories/Research Area,Product Categories/Antibodies,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-1:1000, IHC-P: 1:100-1:500, IF: 1:100-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD279. The exact sequence is proprietary.
• Target: PDCD1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-PD1 antibody, anti-Programmed cell death protein 1 antibody, anti-Protein PD-1 antibody, anti-hPD-1 antibody, anti-CD279 antibody

Western blot analysis of CD279 expression in Raji (A), mouse spleen (B), rat thymus (C) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 50 kD)

Immunofluorescent analysis of CD279 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

PDCD1

UniProt

Q02242, Q15116

NCBI Gene ID

18566, 5133