Anti-CD3 Antibody

SKU: orb248908

Description

Mouse monoclonal antibody to CD3

Research Area

Immunology,Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: FC, IF
• Reactivity: Human
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: OKT3
• Immunogen: Native purified human CD3.
• Target: CD3D; CD3E; CD3G; CD247
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG2a. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CD 3 antibody, anti Cd247 antibody, anti CD247 antigen antibody, anti CD247 antigen, zeta subunit antibody, anti CD247 molecule antibody, anti CD3 antibody, anti CD3 antigen, delta subunit antibody, anti CD3 delta antibody, anti CD3 epsilon antibody, anti CD3 eta antibody, anti CD3 gamma antibody, anti CD3 molecule delta polypeptide antibody, anti CD3 molecule, epsilon polypeptide antibody, anti CD3 molecule, gamma polypeptide antibody, anti CD3 zeta antibody, anti CD3-DELTA antibody, anti CD3D antibody, anti CD3D antigen delta polypeptide antibody, anti CD3d antigen, delta polypeptide (TiT3 complex) antibody, anti CD3d molecule delta antibody, anti CD3d molecule delta CD3 TCR complex antibody, anti CD3d molecule, delta (CD3-TCR complex) antibody, anti CD3E antibody, anti CD3e antigen antibody, anti CD3E antigen epsilon polypeptide antibody, anti CD3e antigen, epsilon polypeptide (TiT3 complex) antibody, anti CD3e molecule epsilon antibody, anti CD3e molecule epsilon CD3 TCR complex antibody, anti CD3e molecule, epsilon (CD3-TCR complex) antibody, anti CD3epsilon antibody, anti CD3G antibody, anti CD3g antigen antibody, anti CD3G antigen gamma polypeptide antibody, anti CD3g antigen, gamma polypeptide (TiT3 complex) antibody, anti CD3g molecule gamma antibody, anti CD3g molecule gamma CD3 TCR complex antibody, anti CD3g molecule, gamma (CD3-TCR complex) antibody, anti CD3H antibody, anti CD3Q antibody, anti CD3Z antibody, anti CD3zeta antibody, anti Ctg3 antibody, anti IMD19 antibody, anti Leu-4 antibody, anti OKT3, delta chain antibody, anti T cell receptor antibody, anti T cell receptor T3 delta chain antibody, anti T cell receptor T3 gamma chain antibody, anti T cell receptor T3 zeta chain antibody, anti T cell receptor zeta chain antibody, anti T cell surface antigen antibody, anti T cell surface antigen T3/Leu 4 epsilon chain antibody, anti T cell surface glycoprotein CD3 antibody, anti T cell surface glycoprotein CD3 delta chain antibody, anti T cell surface glycoprotein CD3 epsilon chain antibody, anti T cell surface glycoprotein CD3 gamma chain antibody, anti T cell surface glycoprotein CD3 zeta chain antibody, anti T-cell antigen receptor complex, delta subunit of T3 antibody, anti T-cell antigen receptor complex, epsilon subunit of T3 antibody, anti T-cell antigen receptor complex, gamma subunit of T3 antibody, anti T-cell antigen receptor complex, zeta subunit of CD3 antibody, anti T-cell receptor T3 delta chain antibody, anti T-cell receptor T3 gamma chain antibody, anti T-cell surface antigen T3/Leu-4 epsilon chain antibody, anti T-cell surface glycoprotein CD3 delta chain antibody, anti T-cell surface glycoprotein CD3 gamma chain antibody, anti T3 antibody, anti T3D antibody, anti T3E antibody, anti T3G antibody, anti T3Z antibody, anti TCRE antibody, anti TCRk antibody, anti TCRZ antibody, anti TCRzeta antibody

Flow cytometric analysis of human peripheral blood lymphocytes using Anti-CD3 Antibody, followed by anti-mouse IgG PE.

Quick Database Links

Gene Symbol

CD3D; CD3E; CD3G; CD247

UniProt

P09693, P04234, P20963, P07766

NCBI Gene ID

916, 917, 915, 919