Anti-CD3 epsilon (activation epitope) Purified

SKU: orb44679

Description

Mouse monoclonal antibody to CD3

Research Area

Epigenetics, Immunology, Stem Cells

Images & Validation

• Tested Applications: FC, ICC, IHC-Fr, IP, WB
• Reactivity: Human, Mouse
• Application Notes:
  Flow cytometry: Intracellular staining; recommended dilution: 1-4 µg/ml; positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/ml). Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS. Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: APA1/1
• Immunogen: Purified human CD3 proteins isolated from thymus
• Target: CD3 epsilon (activation epitope)
• Purity: Purified by protein-A affinity chromatography.
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CD 3 antibody, anti Cd247 antibody, anti CD247 antigen antibody, anti CD247 antigen, zeta subunit antibody, anti CD247 molecule antibody, anti CD3 antibody, anti CD3 antigen, delta subunit antibody, anti CD3 delta antibody, anti CD3 epsilon antibody, anti CD3 eta antibody, anti CD3 gamma antibody, anti CD3 molecule delta polypeptide antibody, anti CD3 molecule, epsilon polypeptide antibody, anti CD3 molecule, gamma polypeptide antibody, anti CD3 zeta antibody, anti CD3-DELTA antibody, anti CD3D antibody, anti CD3D antigen delta polypeptide antibody, anti CD3d antigen, delta polypeptide (TiT3 complex) antibody, anti CD3d molecule delta antibody, anti CD3d molecule delta CD3 TCR complex antibody, anti CD3d molecule, delta (CD3-TCR complex) antibody, anti CD3E antibody, anti CD3e antigen antibody, anti CD3E antigen epsilon polypeptide antibody, anti CD3e antigen, epsilon polypeptide (TiT3 complex) antibody, anti CD3e molecule epsilon antibody, anti CD3e molecule epsilon CD3 TCR complex antibody, anti CD3e molecule, epsilon (CD3-TCR complex) antibody, anti CD3epsilon antibody, anti CD3G antibody, anti CD3g antigen antibody, anti CD3G antigen gamma polypeptide antibody, anti CD3g antigen, gamma polypeptide (TiT3 complex) antibody, anti CD3g molecule gamma antibody, anti CD3g molecule gamma CD3 TCR complex antibody, anti CD3g molecule, gamma (CD3-TCR complex) antibody, anti CD3H antibody, anti CD3Q antibody, anti CD3Z antibody, anti CD3zeta antibody, anti Ctg3 antibody, anti IMD19 antibody, anti Leu-4 antibody, anti OKT3, delta chain antibody, anti T cell receptor antibody, anti T cell receptor T3 delta chain antibody, anti T cell receptor T3 gamma chain antibody, anti T cell receptor T3 zeta chain antibody, anti T cell receptor zeta chain antibody, anti T cell surface antigen antibody, anti T cell surface antigen T3/Leu 4 epsilon chain antibody, anti T cell surface glycoprotein CD3 antibody, anti T cell surface glycoprotein CD3 delta chain antibody, anti T cell surface glycoprotein CD3 epsilon chain antibody, anti T cell surface glycoprotein CD3 gamma chain antibody, anti T cell surface glycoprotein CD3 zeta chain antibody, anti T-cell antigen receptor complex, delta subunit of T3 antibody, anti T-cell antigen receptor complex, epsilon subunit of T3 antibody, anti T-cell antigen receptor complex, gamma subunit of T3 antibody, anti T-cell antigen receptor complex, zeta subunit of CD3 antibody, anti T-cell receptor T3 delta chain antibody, anti T-cell receptor T3 gamma chain antibody, anti T-cell surface antigen T3/Leu-4 epsilon chain antibody, anti T-cell surface glycoprotein CD3 delta chain antibody, anti T-cell surface glycoprotein CD3 gamma chain antibody, anti T3 antibody, anti T3D antibody, anti T3E antibody, anti T3G antibody, anti T3Z antibody, anti TCRE antibody, anti TCRk antibody, anti TCRZ antibody, anti TCRzeta antibody

Separation of Jurkat cells stained anti-human CD3 activation epitope (APA1/1) purified antibody (concentration in sample 5 µg/ml, GAM FITC, red-filled) from Jurkat cells unstained by primary antibody (GAM FITC, black-dashed) in flow cytometry analysis (intracellular staining).

Quick Database Links

Gene Symbol

CD3 epsilon (activation epitope)

UniProt

P07766

NCBI Gene ID

916