CD3 epsilon Rabbit Monoclonal Antibody

SKU: orb548078

Description

Anti-CD3 epsilon Rabbit Monoclonal Antibody. Tested in IHC, ICC/IF, IP applications. This antibody reacts with Human.

Images & Validation

• Tested Applications: ICC, IF, IHC, IP
• Reactivity: Human
• Application Notes:
  IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: COB-3
• Immunogen: A synthesized peptide derived from human CD3 epsilon
• Molecular Weight: 250 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3E; T3E

Immunohistochemical analysis of paraffin embedded mouse spleen labelling CD3 epsilon (orb548078) CD3E was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD3E Antibody (orb548078) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Quick Database Links

UniProt

P07766