CD3 epsilon Rabbit Monoclonal Antibody

SKU: orb548079

Description

Anti-CD3 epsilon Rabbit Monoclonal Antibody. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human.

Images & Validation

• Tested Applications: FC, IHC, IP, WB
• Reactivity: Human
• Application Notes:
  WB 1:500-1:2000IHC 1:50-1:200IP 1:30FC 1:100. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: EED-3
• Immunogen: A synthesized peptide derived from human CD3 epsilon
• Molecular Weight: 250 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e; CD3E; T3E

Western blot analysis of CD3 epsilon expression in Jurkat cell lysate (orb548079). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3E monoclonal antibody (Catalog # orb548079) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

IHC analysis of CD3 epsilon using anti-CD3 epsilon antibody (orb548079) on human colon cancer. CD3 epsilon was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD3 epsilon Antibody (orb548079) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Quick Database Links

UniProt

P07766