Anti-CD3

SKU: orb1132738

Description

Mouse monoclonal antibody to CD3

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies

Images & Validation

• Tested Applications: FC, IP
• Dilution Range: - Flow Cytometry 1:500 (Positive control: Peripheral Blood Lymphocytes, JURKAT human leukemia T cell line) - Immunoprecipitation: The antibody immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4. - Functional Application: The antibody has strong mitogenic effect on peripheral T lymphocytes; it reacts strongly with gamma delta T lymphocytes.
• Reactivity: Human
• Application Notes:
  - Flow Cytometry 1:500 (Positive control: Peripheral Blood Lymphocytes, JURKAT human leukemia T cell line) - Immunoprecipitation: The antibody immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4. - Functional Application: The antibody has strong mitogenic effect on peripheral T lymphocytes; it reacts strongly with gamma delta T lymphocytes.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Immunogen: Human thymocytes and T lymphocytes
• Purity: Affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only