Anti-CD47 Reference Antibody (lemzoparlimab)

SKU: orb1818232

Description

Recombinant Antibody

Images & Validation

• Tested Applications: ELISA, FC
• Reactivity: Human, Monkey

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: IgG4
• Source/Expression System: CHO Cell
• Protein Sequence: CD47
• Molecular Weight: 145.58
• Endotoxin: < 0.001EU/μg,determined by LAL method.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47, CD47, MER6

The purity of Anti-CD47 Reference Antibody (lemzoparlimab) is more than 100%, determined by SEC-HPLC.

Immobilized Recomvinant human CD47 (c His) at1.5 µg/mL can bind Anti-CD47 Reference Antibody (lemzoparlimab), EC50=0.0163 µg/mL

Anti-CD47 Reference Antibody (lemzoparlimab) FACS Blocking was evaluated using CCRF-CEM. The IC50 was approximately 0.4557 ug/mL.

The phagocytosis ratio lemzoparlimab by CCRF-CEM increased with the increase of antibody concentration, and the phagocytosis Rate (%) reached 43%.

Anti-CD47 Reference Antibody (lemzoparlimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

PANC-1 cells were stained with Anti-CD47 Reference Antibody (lemzoparlimab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC317=0.265 ug/mL

Quick Database Links

UniProt

Q08722