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Description
Research Area
Images & Validation
−| Tested Applications | IF, WB |
|---|---|
| Dilution Range | WB: 1:500-2000, IF/ICC: 1:50-200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Recombinant full length protein of human CD55 |
| Target | CD55 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Anti-CD55 Antibody (monoclonal, 5B9E1) [orb1145781]
ICC, IF, IHC, WB
Human
Mouse
Monoclonal
Unconjugated
10 μg, 100 μgAnti-CD55 Antibody (monoclonal, 9F3A1) [orb1145782]
ICC, IF, IHC, WB
Human
Mouse
Monoclonal
Unconjugated
10 μg, 100 μg

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Western blot analysis of CD55 expression in Hela (A), A549 (B) whole cell lysates. (Predicted band size: 39; 41; 48; 56; 59 kD; Observed band size: 60-70 kD)

Immunofluorescent analysis of CD55 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
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Protocol Information
Anti-CD55 Antibody (orb411966)
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