Anti-CD86 Antibody

SKU: orb377982

Description

Rabbit polyclonal antibody to CD86.

Research Area

Immunology,Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Infectious Diseases

Images & Validation

• Tested Applications: IF, IP, WB
• Dilution Range: WB: 1:500-2000, IF/ICC: 1:50-100, IP: 1:50-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant full length protein of human CD86
• Target: CD86
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD86

Western blot analysis of CD86 expression in mouse thymus (A), mouse spleen (B), rat spleen (C), rat lung (D) whole cell lysates. (Predicted band size: 12; 24; 28; 31; 37 kD; Observed band size: 72 kD)

Immunofluorescent analysis of CD86 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

CD86

UniProt

P42081, P42082

NCBI Gene ID

942, 12524