Anti-CD95 Antibody

SKU: orb248955

Description

Mouse monoclonal antibody to CD95

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: FC, IF
• Reactivity: Human
• Application Notes:
  1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Clone No.: DX2
• Immunogen: Native purified human CD95.
• Target: FAS
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FAS antibody, anti APT1 antibody, anti FAS1 antibody, anti TNFRSF6 antibody, anti Tumor necrosis factor receptor superfamily member 6 antibody, anti Apo-1 antigen antibody, anti Apoptosis-mediating surface antigen FAS antibody, anti FASLG receptor antibody, anti CD antigen CD95 antibody

Quick Database Links

Gene Symbol

FAS

UniProt

P25445

NCBI Gene ID

355