Anti-Cytokeratin 19 Purified

SKU: orb43633

Description

Mouse Monoclonal to Cytokeratin 19.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: ELISA, FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.Immunohistochemistry: Recommended dilution: 5-10 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml, positive control: MCF-7, BA-17, HT-29 cells.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: A53-B/A2
• Immunogen: MCF-7 human breast adenocarcinoma cell line
• Target: Cytokeratin 19
• Purity: Purified by protein-A affinity chromatography.
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Cytokeratin 19 antibody

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 µg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) purified antibody (concentration in sample 3 µg/ml, GAM APC).

Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 19 (A53-B/A2).

Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody A53-B/A2 (orb43633, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1 µg/ml; orange).

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody A53-B/A2 on lysates of HT-29 cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 19 monoclonal antibody A53-B/A2 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for cytokeratin 19 at approximately 40 kDa.

Quick Database Links

Gene Symbol

Cytokeratin 19

UniProt

P08727

NCBI Gene ID

3880