Anti-DCR3 Antibody

SKU: orb665733

Description

Rabbit polyclonal antibody to DCR3

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Cancer

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1-500-1000, IHC-P: 1-50-200, IF: 1-100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DCR3. The exact sequence is proprietary.
• Target: TNFRSF6B
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-DCR3 antibody, anti-TR6 antibody, anti-Tumor necrosis factor receptor superfamily member 6B antibody, anti-Decoy receptor 3 antibody, anti-DcR3 antibody, anti-Decoy receptor for Fas ligand antibody, anti-M68 antibody

Western blot analysis of DCR3 expression in HCT116 (A) whole cell lysates. (Predicted band size: 32 kD; Observed band size: 34 kD)

Immunohistochemical analysis of DCR3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of DCR3 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

TNFRSF6B

UniProt

O95407

NCBI Gene ID

8771