DYNLL1/Pin Rabbit Monoclonal Antibody

SKU: orb548208

Description

DYNLL1/Pin Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: EDA-4
• Immunogen: A synthesized peptide derived from human DYNLL1
• Molecular Weight: 32 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Dynein light chain 1, cytoplasmic; 8 kDa dynein light chain; DLC8; Dynein light chain LC8-type 1; Protein inhibitor of neuronal nitric oxide synthase; PIN; DYNLL1; DLC1, DNCL1, DNCLC1, HDLC1

Western blot analysis of DYNLL1 expression in HeLa cell lysate (orb548208). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DYNLL1 monoclonal antibody (Catalog # orb548208) overnight at 4 °C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for DYNLL1.

Quick Database Links

UniProt

P63167