Anti-EGR Antibody

SKU: orb213880

Description

Rabbit polyclonal antibody to EGR2

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: IF, IH, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human EGR1. The exact sequence is proprietary.
• Target: EGR1; EGR2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti EGR1 antibody, anti KROX24 antibody, anti ZNF225 antibody, anti Early growth response protein 1 antibody, anti EGR-1 antibody, anti AT225 antibody, anti Nerve growth factor-induced protein A antibody, anti NGFI-A antibody, anti Transcription factor ETR103 antibody, anti Transcription factor Zif268 antibody, anti Zinc finger protein 225 antibody, anti Zinc finger protein Krox-24 antibody, anti EGR2 antibody, anti KROX20 antibody, anti E3 SUMO-protein ligase EGR2 antibody, anti AT591 antibody, anti Early growth response protein 2 antibody, anti EGR-2 antibody, anti Zinc finger protein Krox-20 antibody

Western blot analysis of EGR expression in Hela (A), PC3 (B), rat ovary (C), MCF7 (D) whole cell lysates. (Predicted band size: 57; 50 kD; Observed band size: 57 kD)

Immunohistochemical analysis of EGR staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of EGR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

EGR1; EGR2

UniProt

P08152, P11161, P51774, P08154, P18146, P08046

NCBI Gene ID

13654, 1958, 114090, 1959, 24330, 13653