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Anti-gamma-Tubulin GCP2 Purified

SKU: orb154401

Description

Mouse monoclonal antibody to Gamma-tubulin complex component 2 which is also known as TUBGCP2 or SPBC97, is a ubiquitously expressed 902 amino acid protein that localizes to the centrosome and is involved in microtubule nucleatio. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsICC, IHC-P, IP, WB
ReactivityHuman, Mouse, Porcine, Rat
Application Notes
Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG2b kappa
Clone No.GCP2-01
ImmunogenGST-fusion protein containing amino acids 2-194 of mouse GCP2
Targetgamma-Tubulin GCP2
PurityPurified by protein-A affinity chromatography.
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

Anti-GCP-2 antibody, anti-GTCC2 antibody, anti-GTC2 antibody

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Anti-gamma-Tubulin GCP2 Purified

Western blotting analysis of human gamma-tubulin complex component 2 (GCP2) using mouse monoclonal antibody GCP2-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-GCP2 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GCP2 at approximately 95 kDa.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC-P
Immunohistochemistry Paraffin
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ICC
Immunocytochemistry
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IP
Immunoprecipitation
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Anti-gamma-Tubulin GCP2 Purified (orb154401)

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0.1 mg
$ 310.00
DispatchUsually dispatched within 5-10 working days weeks.
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