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Anti-GAPDHS Purified

SKU: orb43877

Description

Mouse Monoclonal to Intra-Acrosomal Protein.

Images & Validation

Tested ApplicationsFC, ICC, WB
ReactivityHuman, Porcine
Application Notes
Immunocytochemistry: Recommended dilution: 10 μg/ml; membrane permeabilization (acetone) is essential. Flow cytometry: Recommended dilution: 3-12 µg/ml. Intraacrosomal staining.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgM
Clone No.Hs-8
ImmunogenFreshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
TargetGAPDHS
PurityPurified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
PurificationPurified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-Intra-Acrosomal Protein (Human antibody, anti-Pig) antibody

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol

Available Sizes

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0.1 mg
$ 320.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days.
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