Anti-GSK3 beta Antibody

SKU: orb1474223

Description

Mouse monoclonal antibody to GSK3 beta

Images & Validation

• Tested Applications: FC, IF, IP, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100)
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant GSK-3β protein fragments expressed in E.coli.
• Target: GSK3B
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B

Western blot analysis of GSK3 beta expression in A549 (A), Hela (B), NIH3T3 (C), rat brain (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)

Immunofluorescent analysis of GSK3 beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

GSK3B

UniProt

Q9WV60, P18266, P49841

NCBI Gene ID

56637, 2932, 84027