Anti-HLA-G Purified Low Endotoxin

SKU: orb154421

Description

Mouse monoclonal antibody to HLA-G which is a member of the MHC class I family that is observed in trophoblasts. It involved in the presentation of foreign antigens to the immune system also participate in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells.

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: ELISA, FC, IHC-Fr
• Dilution Range: Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/ml, incubation: 20 min at 25°C; positive tissue: extravillous cytotrophoblast. ELISA: Recommended dilution: 1 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. The antibody 87G has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody W6/32. Functional application: The antibody 87G blocks interaction of HLA-G with inhibitory receptors.
• Reactivity: Human
• Application Notes:
  Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: 87G
• Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
• Target: HLA-G
• Purity: Purified by protein-A affinity chromatography.
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Separation of HLA-G transfected LCL cells (red-filled) from non-transfected LCL cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-HLA-G (87G) purified antibody (concentration in sample 10 μg/ml, GAM APC).

Quick Database Links

Gene Symbol

HLA-G

UniProt

P17693

NCBI Gene ID

3135