Anti-HSP27 (Phospho-S82) Antibody

SKU: orb338948

Description

Rabbit polyclonal antibody to HSPB1

Research Area

Product Categories/Research Area,Product Categories/Antibodies,Epigenetics,Neuroscience,Product Categories/Products/Antibodies/Primary Antibodies,Product Categories/Products/Antibodies,Infectious Diseases

Images & Validation

• Tested Applications: IH, WB
• Dilution Range: WB: 1:500-1000, IHC-P: 1:100-200
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S82 of human HSP27 protein. The exact sequence is proprietary.
• Target: HSPB1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HSP27 antibody, anti HSP28 antibody, anti Heat shock protein beta-1 antibody, anti HspB1 antibody, anti 28 kDa heat shock protein antibody, anti Estrogen-regulated 24 kDa protein antibody, anti Heat shock 27 kDa protein antibody, anti HSP 27 antibody, anti Stress-responsive protein 27 antibody, anti SRP27 antibody

Western blot analysis of HSP27 (Phospho-S82) expression in A549 (A), HepG2 (B), K562 (C) whole cell lysates. (Predicted band size: 22 kD; Observed band size: 27 kD)

Immunohistochemical analysis of HSP27 (Phospho-S82) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

HSPB1

UniProt

P04792, P14602

NCBI Gene ID

15507, 3315