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Hsp27/HSPB1 Antibody (monoclonal, 3H3)

SKU: orb570310

Description

Anti-Hsp27/HSPB1 Antibody (monoclonal, 3H3). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Images & Validation

Tested ApplicationsFC, ICC, IF, IHC, WB
ReactivityHuman
Application Notes
Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells. Add 0.2ml of distilled water will yield a concentration of 500μg/ml

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.3H3
ImmunogenE.coli-derived human Hsp27/HSPB1 recombinant protein (Position: M1-K205). Human Hsp27 shares 83% amino acid (aa) sequence identity with mouse Hsp27.
Molecular Weight27 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27; HSPB1; HSP27; HSP28

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol

Available Sizes

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100 μg
$ 500.00
Free Secondary Antibody (20 ul)0/0

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DispatchCurrently estimated at 1-3 months
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