Anti-HSP90 beta Antibody

SKU: orb1474297

Description

Mouse monoclonal antibody to HSP90 beta

Images & Validation

• Tested Applications: IF, IP, WB
• Dilution Range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
• Reactivity: Hamster, Human, Mouse, Primate, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: Purified recombinant human Hsp90 beta protein fragments expressed in E.coli
• Target: HSP90AB1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSP90B; HSPC2; HSPCB; Heat shock protein HSP 90-beta; HSP 90; Heat shock 84 kDa; HSP 84; HSP84

Western blot analysis of HSP90 beta expression in Hela (A), NIH3T3 (B), C6 (C), CHOK1 (D), COS7 (E) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 90 kD)

Immunofluorescent analysis of HSP90 beta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

HSP90AB1

UniProt

P11499, P34058, P08238

NCBI Gene ID

301252, 15516, 3326