Anti-Hu BCL2 PE

SKU: orb44690

Description

Mouse monoclonal antibody against BCL2 conjugated to PE

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Intracellular staining.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: Bcl-2/100
• Immunogen: Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2
• Target: BCL2
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-Apoptosis regulator Bcl 2 antibody, anti- Apoptosis regulator BCL2 antibody, anti- Apoptosis regulator BCL2 antibody, anti- AW986256 antibody, anti- B cell CLL/lymphoma 2 antibody, anti- B cell leukemia/lymphoma 2 antibody, anti- BCL2 antibody, anti- BCL2 antibody, anti- BCL2_human antibody, anti- C430015F12Rik antibody, anti- D630044D05Rik antibody, anti- D830018M01Rik antibody, anti- Leukemia/lymphoma, B-cell, 2 antibody, anti- Oncogene B-cell leukemia 2 antibody, anti- PPP1R50 antibody, anti- Protein phosphatase 1, regulatory subunit 50 antibody

Flow cytometry multicolor staining of human lymphocytes stained using anti-human BCL2 (Bcl-2/100) PE antibody (intracellular staining, 10 µl reagent / 100 µl of peripheral whole blood from DLBCL patient) and anti-human CD19 (LT19) FITC antibody (surface staining, 20 µl reagent / 100 µl of peripheral whole blood from DLBCL patient).

Separation of human BCL-2 positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood from DLBCL patient stained using anti-human BCL2 (Bcl-2/100) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry intracellular staining pattern of human peripheral whole blood from diffuse large B-cell lymphoma (DLBCL) patient. Sample was stained using anti-human BCL2 (Bcl-2/100) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human BCL2 (Bcl-2/100) PE antibody (concentration in sample 1.67 µg/ml).

Separation of human BCL2 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human BCL2 (Bcl-2/100) PE antibody (concentration in sample 1.67 µg/ml).

Quick Database Links

Gene Symbol

BCL2

UniProt

P10415

NCBI Gene ID

596