Anti-Hu C3b/iC3b Purified

SKU: orb2321157

Description

Complement component C3 plays a key role in the activation of complement system. In classical complement system pathway the activated C2 and C4 form classical C3-convertase (C4b2b) which cleaves C3 into C3a and C3b. In alternative complement system pathway C3 is cleaved by alternative C3-convertase (C3bBb), composed of C3b (which can be generated also by spontaneous hydrolysis of C3) and the activated form of factor B. C3b activates downstream cascade leading to formation of pores in the plasma membrane of attacked cell. C3b and its proteolytically inactive form iC3b also serve as important opsonins. C3b can generate C3f, as well as the iC3b can be further cleaved into C3c, C3d and C3g. Complement system is regulated by effective inactivation of free C3b by factor H and factor I. C3b attached to the surface of its target is protected from this inactivation. Complement system is also regulated by other proteins, e.g. CD35, CD46, CD55, or CD59. Undesired activation of complement cascade can lead to severe diseases such as PNH, rheumatoid arthritis, macular degeneration and other. Recently it has been demonstrated to take part in complications associated with Covid-19.

Images & Validation

• Tested Applications: ELISA, FC, ICC
• Reactivity: Human, Primate
• Application Notes:
  Flow cytometry: Recommended dilution: 0.5-4 μg/ml.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: 3E7
• Immunogen: human complement component C3b
• Target: C3b/iC3b
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

complement component C3, complement component C3b, complement component iC3b

Quick Database Links

Gene Symbol

C3b/iC3b

UniProt

P01024

NCBI Gene ID

718