Anti-Hu CD294 APC

SKU: orb1881233

Description

CD294 (prostaglandin D2 receptor 2) is a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells, but also on eosinophils and basophils. It mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of CD294 gene have been associated with asthma susceptibility. Outside the immune system CD294 is expressed e.g. in gut, heart, and brain. The intracellular C terminal tail contains sites for phosphorylation by protein kinase C.

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Clonality: Monoclonal
• Isotype: Rat IgG2a
• Clone No.: BM16
• Immunogen: Human CD294 transfected rat cell line TART/B19-12.10
• Target: CD294
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DP2, PTGDR2, DL1R, CRTH2, GPR44

Flow cytometry multicolor surface staining pattern of human blood sample using anti-human CD294 (BM16) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).

Separation of human basophil granulocytes (red-filled) from CD294 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD294 (BM16) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD294 (BM16) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Quick Database Links

Gene Symbol

CD294

UniProt

Q9Y5Y4

NCBI Gene ID

11251