Anti-Hu CD315 Purified

SKU: orb1678983

Description

CD315, also known as prostaglandin F2 receptor negative regulator, is an approximately 135 kDa transmembrane glycoprotein, which associates with actin cytoskeleton, and with CD9 and CD81, but not with other tetraspanins. It seems to be involved in regulation of cell polarity and motility. CD315 is expressed mainly by keratinocytes, activated monocytes, and a subset of B cells, but it can be also used for distinguishing between strongly positive colon cancer and fibrosarcoma cells, and their negative normal cell counterparts.

Images & Validation

• Tested Applications: FC, IHC-Fr, IP, WB
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 µg/ml

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 1F11
• Immunogen: HeLa cells
• Target: CD315
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PF2RI, SMAP-6, PTGFRN, FPRP, EWI-F

Separation of A431 cells stained using anti-human CD315 (1F11) purified antibody (concentration in sample 1.67 μg/ml, GAM APC) from A431 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Quick Database Links

Gene Symbol

CD315

UniProt

Q9P2B2

NCBI Gene ID

5738