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Description
Research Area
Epigenetics, Infectious Diseases
Images & Validation
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Item 1 of 3
| Tested Applications | FC, ICC, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | Immunohistochemistry (frozen sections): Recommended dilution: 5-10 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; prolonged fixation in buffered formalin can destroy the epitope. High temperature antigen unmasking technique is required. Flow cytometry: Recommended dilution: 1-4 μg/ml. Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone No. | 1H4 |
| Immunogen | Raji cells and spleen cells fused with NS1 cells |
| Target | CD54 |
| Purity | Purified by protein-A affinity chromatography. |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-CD54 antibody, anti-ICAM-1 antibody
Quality Guarantee
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
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