Anti-Hu CD79a FITC

SKU: orb1821019

Description

CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

Images & Validation

• Tested Applications: FC
• Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Human, Mouse, Porcine, Primate, Rabbit, Rat
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone No.: HM47
• Immunogen: Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
• Target: CD79a
• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BCR alpha, Ig-alpha, MB-1, IGA

Flow cytometry multicolor intracellular staining pattern of human lymphocytes using anti-human CD79a (HM47) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD19 (LT19) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD19 positive CD79a positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human CD79a (HM47) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human CD79a (HM47) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Quick Database Links

Gene Symbol

CD79a

UniProt

P11912

NCBI Gene ID

973