Anti-Hu CD99 Purified

SKU: orb154388

Description

Mouse monoclonal antibody to CD99 which belongs to the CD99 family. It plays role in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. CD99 is expressed in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation.

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: Recommended dilution: 1-4 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a kappa
• Clone No.: 3B2/TA8
• Immunogen: Human thymocytes
• Target: CD99
• Purity: Purified by protein-A affinity chromatography.
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-MIC2 antibody, anti-HBA71 antibody, anti-blood group Xg antibody

Mass cytometry (surface staining) of human peripheral blood cells (after separation using Ficoll-Paque density gradient centrifugation) with anti-human CD99 (3B2/TA8) Eu153. Gated on singlets.

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD99 (3B2/TA8) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD99

UniProt

P14209

NCBI Gene ID

4267