Anti-Hu p53 (pS392) Purified

SKU: orb43788

Description

Mouse monoclonal antibody to p53 (phospho-Ser392)

Research Area

Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IHC-P, WB
• Reactivity: Human
• Application Notes:
  Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0, recommended dilution: 10 μg/ml, incubation: 1 hour at RT; or overnight at 4°C, positive tissue: breast carcinoma with high level of wild-type p53. Western blotting: recommended dilution: 1 μg/ml.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: FP3.2 [FPS392]
• Immunogen: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.
• Target: p53 (pS392)
• Purity: Purified by protein-A affinity chromatography.
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti BCC7 antibody, anti FLJ92943 antibody, anti HGNC11998 antibody, anti LFS1 antibody, anti p53 antibody, anti p53 Cellular Tumor Antigen antibody, anti p53 tumor suppressor antibody, anti Phosphoprotein p53 antibody, anti Tp53 antibody, anti TRP53 antibody, anti Tumor protein 53 antibody, anti Tumor protein p53 antibody, anti TP53 antibody

Quick Database Links

Gene Symbol

p53 (pS392)

UniProt

P04637

NCBI Gene ID

7157