Anti-Hu SIGLEC8 PE

SKU: orb1147807

Description

SIGLEC8 is a sialic acid binding lectin similar to CD33. In its cytoplasmic comain it contains an immunoreceptor tyrosine-based inhibitory motif (ITIM), and a motive similar to a binding site for SLAM-associated protein. SIGLEC8 is expressed e.g. in lymph nodes and spleen. It is an eosinophil marker, although it can be found also on the surface of mast cells. Crosslinking of SIGLEC8 leads to apoptosis. Soluble form of SIGLEC8 can be foud in human serum, especially in case of eosinophil-associated diseases.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Epigenetics

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 7C9
• Immunogen: Extracellular domain of human SIGLEC8 fused with Fc fragment of human IgG1
• Target: SIGLEC8
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SAF2

Quick Database Links

Gene Symbol

SIGLEC8

UniProt

Q9NYZ4

NCBI Gene ID

27181