Anti-Hu TCR gamma/delta APC

SKU: orb1742785

Description

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 11F2
• Immunogen: Purified CD3 antigen complex
• Target: TCR gamma/delta
• Conjugation: APC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TCRG/D

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood) and anti-human TCR gamma/delta (11F2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 positive TCR gamma/delta positive lymphocytes (red-filled) from CD3 negative TCR gamma/delta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Quick Database Links

Gene Symbol

TCR gamma/delta