Anti-Hu TCR gamma/delta PE

SKU: orb1146447

Description

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Research Area

Product Categories/Products/Antibodies

Images & Validation

• Tested Applications: FC
• Reactivity: Human
• Application Notes:
  Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: 11F2
• Immunogen: Purified CD3 antigen complex
• Target: TCR gamma/delta
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TCRG/D

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR gamma/delta (11F2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human TCR gamma/delta positive T cellls (red-filled) from TCR gamma/delta negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Quick Database Links

Gene Symbol

TCR gamma/delta