Anti-KEAP1 Antibody

SKU: orb1474440

Description

Rabbit monoclonal antibody to KEAP1

Images & Validation

• Tested Applications: IH, WB
• Dilution Range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: Recombinant protein of human Keap1
• Target: KEAP1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19

Western blot analysis of KEAP1 expression in Raw264.7 (A), Hela (B), A549 (C), C2C12 (D) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 60-64 kD)

Immunohistochemical analysis of KEAP1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.22). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

KEAP1

UniProt

Q14145, Q9Z2X8

NCBI Gene ID

9817, 50868